Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)
![Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)](https://www.abcam.com/ps/products/253/ab253243/Images/ab253243-1-253243ICC1.jpg "Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22538-206] to ARMET/ARP
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-ARMET/ARP antibody [EPR22538-206]
See all ARMET/ARP primary antibodies -
Description
Rabbit monoclonal [EPR22538-206] to ARMET/ARP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa cells. Flow Cyt: HeLa cells. WB: HeLa and HT-1080 whole cell lysate. His-tagged human ARMET/ARP recombinant protein. IP: HeLa whole cell lysate.
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General notes
This product was previously labelled as ARMET
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22538-206 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ARMET/ARP with ab253243 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in HeLa cell line. The nuclear counterstain is DAPI (blue). Counterstained with ab12223 Anti-KDEL antibody - Endoplasmic reticulum Marker (Mouse monoclonal) ab150120 AlexaFluor®594 Goat anti-Mouse secondary.
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Flow Cytometry - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)](https://www.abcam.com/ps/products/253/ab253243/Images/ab253243-4-253243FC1.jpg)
Flow Cytometry - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling ARMET/ARP with ab253243 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)](https://www.abcam.com/ps/products/253/ab253243/Images/ab253243-2-253243WB1.jpg)
Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)All lanes : Anti-ARMET/ARP antibody [EPR22538-206] (ab253243) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 59 secondsThe expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 22637475).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)](https://www.abcam.com/ps/products/253/ab253243/Images/ab253243-3-253243WB2.jpg)
Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)All lanes : Anti-ARMET/ARP antibody [EPR22538-206] (ab253243) at 1/1000 dilution
Lane 1 : His-tagged human ARMET/ARP recombinant protein 20 ng
Lane 2 : His-tagged human CDNF recombinant protein 20 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsNo cross reactivity was found for CDNF.
Blocking/Dilution: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)](https://www.abcam.com/ps/products/253/ab253243/Images/ab253243-5-253243IP1.jpg)
Immunoprecipitation - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)ARMET/ARP was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab253243 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab253243 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab253243 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253243 in HeLa whole cell lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 15 seconds. -
![Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)](https://www.abcam.com/ps/products/253/ab253243/Images/ab253243-6-benefits-of-recombinant-antibodies.png)
Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)
