Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22538-206] to ARMET/ARP
Suitable for: WB, ICC/IF, Flow Cyt, IP
Reacts with: Human

Product name

Anti-ARMET/ARP antibody [EPR22538-206]
See all ARMET/ARP primary antibodies
Description

Rabbit monoclonal [EPR22538-206] to ARMET/ARP
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC/IF: HeLa cells. Flow Cyt: HeLa cells. WB: HeLa and HT-1080 whole cell lysate. His-tagged human ARMET/ARP recombinant protein. IP: HeLa whole cell lysate.
General notes
This product was previously labelled as ARMET

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22538-206
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ARMET/ARP with ab253243 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in HeLa cell line. The nuclear counterstain is DAPI (blue). Counterstained with ab12223 Anti-KDEL antibody - Endoplasmic reticulum Marker (Mouse monoclonal) ab150120 AlexaFluor^®594 Goat anti-Mouse secondary.

Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling ARMET/ARP with ab253243 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

All lanes : Anti-ARMET/ARP antibody [EPR22538-206] (ab253243) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 20 kDa
Observed band size: 18 kDa
why is the actual band size different from the predicted?

Exposure time: 59 seconds

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 22637475).

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

All lanes : Anti-ARMET/ARP antibody [EPR22538-206] (ab253243) at 1/1000 dilution

Lane 1 : His-tagged human ARMET/ARP recombinant protein 20 ng
Lane 2 : His-tagged human CDNF recombinant protein 20 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

No cross reactivity was found for CDNF.

Blocking/Dilution: 5% NFDM/TBST.
Immunoprecipitation - Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

ARMET/ARP was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab253243 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab253243 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab253243 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253243 in HeLa whole cell lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 15 seconds.
Anti-ARMET/ARP antibody [EPR22538-206] (ab253243)

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