Histone Acetyltransferase Inhibitor Screening Assay Kit (ab133099)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
Histone Acetyltransferase Inhibitor Screening Assay Kit
See all Histone acetyltransferase kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% HAT 16 3.2% Inter-assay Sample n Mean SD CV% HAT 16 4.4% -
Assay type
Enzyme activity -
Product overview
Histone Acetyltransferase Inhibitor Screening Kit (ab133099) provides a fast, fluorescence-based method for evaluating pCAF HAT inhibitors.
The procedure is based on the ability of 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin (CPM) to react with free thiol grious present after the acetylation reaction. The reaction of the CPM reacts these free thiol groups forms a highly fluorescent product that is detected using excitation and emission wavelengths of 360-390 nm and 450-470 nm, respectively.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests 96-Well Plate (white) 1 unit 96-Well Plate Cover 1 unit HAT Acetyl CoA 1 vial HAT Assay Buffer (5X) 1 vial HAT Developer 1 vial HAT Peptide 1 vial HAT Stop Reagent 1 vial Histone Acetyltransferase (pCAF) 1 vial -
Research areas
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Relevance
Histone acetyltransferases (HAT) are enzymes that acetylate conserved lysine amino acids on histone proteins. They have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. -
Cellular localization
Cytoplasmic and Nuclear. Note=Nuclear in S-phase cells and cytoplasmic. -
Alternative names
- HAT
- HAT1
- Histone acetyltransferase 1
see all
Images
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Effect of garcinol in HAT activity.An example of a known pCAF HAT inhibitor, garcinol, interfering with the assay.
