Histone Acetyltransferase (HAT) Activity Assay Kit (Fluorometric, Histone H4) (ab239713)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Cell culture media, Cell culture supernatant, Cell Lysate, Tissue
Overview
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Product name
Histone Acetyltransferase (HAT) Activity Assay Kit (Fluorometric, Histone H4)
See all Histone acetyltransferase kits -
Detection method
Fluorescent -
Sample type
Cell culture supernatant, Cell culture extracts, Tissue, Cell Lysate, Cell culture media -
Product overview
Histone Acetyltransferase (HAT) Activity Assay Kit (Fluorometric, Histone H4) (ab239713) utilizes Acetyl CoA and H4 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer and Probe to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm.
The assay can detect HAT activity equivalent to as low as 1.5 U of p300 in a variety of samples.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 100 tests Acetyl CoA (Lyophilized) 1 vial CoA Standard (Lyophilized) 1 vial Developer 1 x 100µl H4 Peptide (Lyophilized) 1 vial HAT Assay Buffer 1 x 25ml Positive Control (HeLa Nuclear Extract) 1 x 40µl Probe 1 x 200µl Substrate Mix (Lyophilized) 1 vial -
Research areas
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Relevance
Histone acetyltransferases (HAT) are enzymes that acetylate conserved lysine amino acids on histone proteins. They have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. -
Cellular localization
Cytoplasmic and Nuclear. Note=Nuclear in S-phase cells and cytoplasmic. -
Alternative names
- HAT
- HAT1
- Histone acetyltransferase 1
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Images
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Measurement of HAT Activity: p300 (72 ng) or HeLa nuclear extract (positive control)
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CoASH Standard Curve
