Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Nuclear Extracts, Purified protein
Overview
-
Product name
Histone Acetyltransferase Activity Assay Kit (Fluorometric)
See all Histone acetyltransferase kits -
Detection method
Fluorescent -
Sample type
Nuclear Extracts, Purified protein -
Assay type
Enzyme activity -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709) utilizes Acetyl CoA and H3 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from AcetylCoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity as low as 6 mU in a variety of samples.
-
Notes
Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases.
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 100 tests Acetyl CoA (Lyophilized) 1 vial CoA Standard (Lyophilized) 1 vial Developer 1 x 100µl H3 Peptide (Lyophilized) 1 vial HAT Assay Buffer 1 x 25ml PicoProbe 1 x 200µl Positive Control (HeLa Nuclear Extract) 1 x 40µl Substrate Mix (Lyophilized) 1 vial -
Research areas
-
Relevance
Histone acetyltransferases (HAT) are enzymes that acetylate conserved lysine amino acids on histone proteins. They have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. -
Cellular localization
Cytoplasmic and Nuclear. Note=Nuclear in S-phase cells and cytoplasmic. -
Alternative names
- HAT
- HAT1
- Histone acetyltransferase 1
see all
Images
-
Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)
Typical CoA Standard calibration curve.
-
Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)HAT Activity in HeLa Nuclear Extract.
-
Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)Specific Activity of HeLa Nuclear Extract and purified recombinant pCAF.
