Human MFGE8 Antibody Pair - BSA and Azide free (ab244126)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human MFGE8 Antibody Pair - BSA and Azide free
See all Milk Fat Globule 1 kits -
Assay type
ELISA set -
Range
15.6 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human MFGE8. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab235638), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human MFGE8 Capture Antibody (unconjugated) 1 x 100µg Human MFGE8 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
Medin is the main constituent of aortic medial amyloid. -
Tissue specificity
Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas. -
Sequence similarities
Contains 1 EGF-like domain.
Contains 2 F5/8 type C domains. -
Post-translational
modificationsMedin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273. -
Cellular localization
Membrane. Secreted. - Information by UniProt
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Alternative names
- BA46
- Breast epithelial antigen BA46
- EDIL1
see all -
Database links
- Entrez Gene: 4240 Human
- Omim: 602281 Human
- SwissProt: Q08431 Human
- Unigene: 3745 Human
Images
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Sandwich ELISA - Human MFGE8 Antibody Pair - BSA and Azide free (ab244126)To learn more about the advantages of recombinant antibodies see here.
