Human MFGE8 ELISA Kit (ab235638)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.4 pg/ml
- Range: 15.6 pg/ml - 2000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, Milk, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MFGE8 ELISA Kit
See all Milk Fat Globule 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 5 4.3% Inter-assay Sample n Mean SD CV% Supernatant 3 8.2% -
Sample type
Cell culture supernatant, Milk, Serum, Cell culture extracts, Tissue Extracts, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.4 pg/ml -
Range
15.6 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 103 91% - 114% Milk 86 80% - 91% Serum 124 121% - 128% Cell culture extracts 91 89% - 94% Tissue Extracts 93 88% - 98% Cell culture media 94 88% - 98% Cit plasma 121 117% - 124% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human MFGE8 ELISA Kit (ab235638) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MFGE8 protein in cell culture supernatant, cit plasma, milk, serum, tissue extracts, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MFGE8 with 2.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MFGE8 Capture Antibody 1 x 600µl 10X Human MFGE8 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human MFGE8 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
Medin is the main constituent of aortic medial amyloid. -
Tissue specificity
Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas. -
Sequence similarities
Contains 1 EGF-like domain.
Contains 2 F5/8 type C domains. -
Post-translational
modificationsMedin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273. -
Cellular localization
Membrane. Secreted. - Information by UniProt
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Alternative names
- BA46
- Breast epithelial antigen BA46
- EDIL1
see all -
Database links
- Entrez Gene: 4240 Human
- Omim: 602281 Human
- SwissProt: Q08431 Human
- Unigene: 3745 Human
Images
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Other - Human MFGE8 ELISA Kit (ab235638)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human MFGE8 Competitor Std Curve ComparisonStandard curve comparison between human MFGE8 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human MFGE8 standard curve in Sample Diluent 10BSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of human MFGE8 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of human MFGE8 standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike and native MFGE8 in human serum and plasma (citrate)The concentrations of MFGE8 were measured in duplicates, interpolated from the MFGE8 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25% and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean native MFGE8 concentration was determined to be 694.7 pg/mL in neat serum and 178.1 pg/mL in neat plasma (citrate).
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Interpolated concentrations of native MFGE8 in human milk and A549 cell culture supernatant samplesThe concentrations of MFGE8 were measured in duplicates, interpolated from the MFGE8 standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 1:20,000 and A549 cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MFGE8 concentration was determined to be 6794 ng/mL in neat milk and 7.260 ng/mL in neat A549 cell culture supernatant.
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Interpolated concentrations of native MFGE8 in human A549 cell extract, human liver tissue extract and human placenta tissue extractInterpolated concentrations of native MFGE8 in human A549 cell extract based on a 25 µg/mL extract load, human liver tissue extract based on a 400 µg/mL extract load, and human placenta tissue extract based on a 200 μg/mL extract load. The concentrations of MFGE8 were measured in duplicate and interpolated from the MFGE8 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MFGE8 concentration was determined to be 756.6 pg/mL in A549 cell extract, 230.1 pg/mL in human liver tissue extract, and 658.5 pg/mL in human placenta tissue extract.
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MFGE8 concentration was determined to be 260.5 pg/mL with a range of 98.60 – 406.6 pg/mL.
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Comparison of native human MFGE8 in cell and tissue extracts expressed in ng of MFGE8 per mg of extractThe concentrations of MFGE8 were measured in duplicate and interpolated from the MFGE8 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MFGE8 concentration was determined to be 29.24 ng/mg in A549 cell extract, 0.546 ng/mg in human liver tissue extract, and 3.222 ng/mg in human placenta tissue extract.
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Sandwich ELISA - Human MFGE8 ELISA Kit (ab235638)To learn more about the advantages of recombinant antibodies see here.
