Antibodies, Proteins, Kits and Reagents for Life Science

Human MFGE8 ELISA Kit (Lactadherin) (ab213810)

Key features and details

Sensitivity: 10 pg/ml
Range: 62.5 pg/ml - 4000 pg/ml
Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

Storage instructions

Store at -20°C. Please refer to protocols.

Components	Identifier	1 x 96 tests
ABC Diluent Buffer	Blue Cap	1 x 12ml
Adhesive Plate Seal		4 units
Antibody Diluent Buffer	Green Cap	1 x 12ml
Anti-Human MFGE8 coated Microplate (12 x 8 wells)		1 unit
Avidin-Biotin-Peroxidase Complex (ABC)		1 x 100µl
Biotinylated anti-Human MFGE8 antibody		1 x 130µl
Lyophilized recombinant Human MFGE8 standard		2 vials
Sample Diluent Buffer	Green Cap	1 x 30ml
TMB Color Developing Agent	Black Cap	1 x 10ml
TMB Stop Solution	Yellow Cap	1 x 10ml

Research areas
Function

Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
Medin is the main constituent of aortic medial amyloid.
Tissue specificity

Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
Sequence similarities

Contains 1 EGF-like domain.
Contains 2 F5/8 type C domains.
Post-translational
modifications

Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
Cellular localization

Membrane. Secreted.
Target information above from: UniProt accession Q08431 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Alternative names
- BA46
- Breast epithelial antigen BA46
- EDIL1
- HMFG
- hP47
- HsT19888
- Lactadherin
- Medin
- MFG-E8
- MFGE8
- MFGM
- MFGM_HUMAN
- Milk fat globule EGF factor 8
- Milk fat globule EGF factor 8 protein
- Milk fat globule-EGF factor 8
- O acetyl disialoganglioside synthase
- OAcGD3S
- SED1
- SPAG10
- Sperm associated antigen 10
- Sperm surface protein hP47
see all
Database links
- Entrez Gene: 4240 Human
- Omim: 602281 Human
- SwissProt: Q08431 Human
- Unigene: 3745 Human

Human MFGE8 ELISA Kit (Lactadherin) (ab213810) Standard Curve

Human MFGE8 ELISA Kit (Lactadherin) (ab213810) Standard Curve

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