Goat F(ab) Anti-Mouse IgG H&L (ab6668)
Key features and details
- Goat F(ab) Anti-Mouse IgG H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ICC/IF, IHC, ELISA
Overview
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Product name
Goat F(ab) Anti-Mouse IgG H&L
See all IgG secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC, ELISAmore details -
Immunogen
Mouse IgG whole molecule
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).” -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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SDS-PAGE - Goat F(ab) Anti-Mouse IgG H&L (ab6668)
SDS-PAGE results of Goat Fab Anti-Mouse IgG (H&L) ab6668.
Lane 1: Non-reduced Goat Fab Anti-Mouse.
Lane 2: Opal Prestained Ladder.
Lane 3: Reduced Goat Fab Anti-Mouse.
Load: 1.0 µg.
Coomassie stained and imaged.
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Dot Blot - Goat F(ab) Anti-Mouse IgG H&L (ab6668)Dot Blot results of Goat Fab Anti-Mouse IgG Antibody ab6668.
Dots are Mouse IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng.
Blocked for 30 min at RT.
Primary Antibody: Goat Fab Anti-Mouse IgG at 1 µg/mL for 1 hr at RT.
Secondary Antibody: Donkey Anti-Goat IgG HRP at 1:40,000 for 30 min at RT.
Imaged with BioRad ChemiDoc, Chemi filter.
