Donkey Anti-Sheep IgG H&L (DyLight® 650) (ab96942)
Key features and details
- Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 650)
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Flow Cyt, IHC-P, ICC/IF
Overview
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Product name
Donkey Anti-Sheep IgG H&L (DyLight® 650)
See all IgG secondary antibodies -
Description
Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 650) -
Host species
Donkey -
Target species
Sheep -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
Tested applications
Suitable for: Flow Cyt, IHC-P, ICC/IFmore details -
Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 650 replaces DyLight® 649. -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (DyLight® 650) (ab96942) This image is courtesy of an Abreview submitted by Marcella Flores.
ab62116 staining MAP1LC3A in mouse splinocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 2% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1 µg/ml in PBS/2% FBS) for 24 hour at 4°C. A DyLight® 650-conjugated donkey anti-sheep IgG H&L (ab96942) (1/1000) was used as the secondary antibody.
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Donkey Anti-Sheep IgG H&L (DyLight® 650) (ab96942)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.