Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)
Key features and details
- Goat Anti-Rabbit IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Fr
Overview
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Product name
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
Immunogen
Full length native Rabbit IgG (purified).
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) Lean QY et al. PLoS One (2015) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunohistochemical analysis of healthy and colitis mouse colon sections (untreated and treated with enoxaparin) labeling claudin-4 with ab15104 at 1/200. ab7090, anti-rabbit immunoglobulin G conjugated to horseradish peroxidase (HRP) at 1/300 was used as the secondary antibody. ab64238 at 1/50 was used to develop the histological signal.
Antigen retrieval was performed by incubating the sections for 10 minutes at 97°C in 1 mM EDTA buffer, pH 8.0 or 10 mM citrate buffer, pH 6.0.
Control, C; untreated colitis, DSS; oral enoxaparin, OE; intraperitoneal injection of enoxaparin, IPE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) This image is courtesy of an anonymous Abreview.ab9986 staining osteoprotegerin in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA and 5% normal goat serum in PBS for 30 minutes at 22°C; Samples were incubated with primary antibody (1/20) for 9 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG H&L (ab7090) (1/300) was used as the secondary antibody.
