FITC Anti-Human IgG antibody (ab81051)
Key features and details
- FITC Goat polyclonal to Human IgG
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-Human IgG antibody
See all Human IgG primary antibodies -
Description
FITC Goat polyclonal to Human IgG -
Host species
Goat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
Low background fluorescence of normal tissue in contrast to cases of autoimmune disease, deposited immunoglobulin IgG. No cross reactivity with albumin fractions of human serum. No cross reactivity with albumin fractions of animal species. -
Species reactivity
Reacts with: Human -
Immunogen
Human IgG.
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General notes
The purified polyclonal was conjugated to fluorescein isothiocyanate isomer I followed by gel filtation and ion exchange chromatography to clear unbound conjugate. F/P ratio: 4-6 mol Fluorescein per 1 mol of goat IgG.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.50
Constituents: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorbed by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralisation and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
