Anti-Human IgG antibody [EPR12700] (ab181236)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12700] to Human IgG
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Human
Overview
-
Product name
Anti-Human IgG antibody [EPR12700]
See all Human IgG primary antibodies -
Description
Rabbit monoclonal [EPR12700] to Human IgG -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Full length native protein (purified) corresponding to Human Human IgG. Isolated from a pool of normal Human sera.
-
Positive control
- Human plasma, thymus, spleen and tonsil tissue lysates; Human IgG; Human spleen and colon tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12700 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Human IgG with purified ab181236 at 1/100 dilution (1.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
-
Anti-Human IgG antibody [EPR12700] (ab181236) at 1/10000 dilution (purified) + Human tonsil lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 36, 41 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
-
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Human IgG with unpurified ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
-
Anti-Human IgG antibody [EPR12700] (ab181236) at 1/2000 dilution (purified) + Human spleen lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 36, 41 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST.
-
All lanes : Anti-Human IgG antibody [EPR12700] (ab181236) at 1/10000 dilution (unpurified)
Lane 1 : Human plasma lysate
Lane 2 : Human thymus lysate
Lane 3 : Human tonsil lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 36, 41 kDa
-
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Human IgG with unpurified ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
-
All lanes : Anti-Human IgG antibody [EPR12700] (ab181236) at 1/2000 dilution (unpurified)
Lane 1 : Human IgG
Lane 2 : Human IgM
Lane 3 : Human IgA
Lane 4 : Human IgD
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36, 41 kDa
-