The Goat Anti-Mouse IgG antibody (GAM) is covalently conjugated to 20 nm Gold Nanoparticles which have a unique protective coating that can withstand most extreme conditions (e.g. 2.5 M NaOH at 70°C for >1 hour). The irreversible attachment does not allow the desorption of the antibody, which is a major problem with products in which the antibody is only passively adsorbed to the metal.

To exchange the conjugated antibody into a different buffer specific to your application, centrifuge the conjugate in a microfuge at 9,000 x g 20 minutes. Carefully remove the supernatant, gently tap the pellet and add your preferred buffer. It is important to avoid substances that have a very high affinity for gold (e.g. thiols).

If an incubation of Goat Anti-Mouse IgG (20nm Gold, 10 OD) with the target antibody is performed, do not centrifuge the gold complex after this stage (an antibody titration is recommended).

The table below is just a guideline for possible applications:

This product is manufactured by Expedeon, an Abcam company. It was previously called 20nm InnovaCoat^® GOLD (10 OD) Goat Anti Mouse IgG Conjugate. Expedeon product code 215-0200 is the same as the 200 µl size of this product and code 215-1000 is the same as the 1 mL size.