IHC image of Human IgG staining in a section of formalin-fixed paraffin-embedded normal human tonsil and normal human cerebral cortex, performed on a Leica BOND^TM system using the standard protocol F. The tissue sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with ab213242, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system.

DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Tonsil tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Different concentrations of immobilised human IgG (x-axis) was recognised in an indirect ELISA by ab213242 (1 µg x mL^-1). Bound ab213242 was detected by peroxidase conjugated goat anti-rabbit IgG (1.6 µg x mL^-1). OD (background signal subtracted) shown on the y-axis.

IHC image of Human IgG staining in a section of formalin-fixed paraffin-embedded normal human tonsil, performed on a Leica BOND^TM system using the standard protocol F. The tissue section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab213242, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system.

DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Tonsil tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC image of Human IgG staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex, performed on a Leica BOND^TM system using the standard protocol F. The tissue section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab213242, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system.

DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.