Anti-IFNGR1 (phospho Y457) antibody (ab61062)
Key features and details
- Rabbit polyclonal to IFNGR1 (phospho Y457)
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-IFNGR1 (phospho Y457) antibody
See all IFNGR1 primary antibodies -
Description
Rabbit polyclonal to IFNGR1 (phospho Y457) -
Host species
Rabbit -
Specificity
ab61062 detects endogenous levels of IFNGR1 only when phosphorylated at tyrosine 457. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB African green monkey
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Immunogen
Synthetic phosphopeptide derived from human IFNGR1 around the phosphorylation site of tyrosine 457 (GYpDKP).
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Positive control
- Human brain tissue, COS7 cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab61062 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFNGR1 (phospho Y457) antibody (ab61062)Immunohistochemistry analysis of paraffin-embedded human brain tissue using IFNGR1 (phospho Y457) antibody (ab61062) at 1/50 - 1/100 dilution, in the presence (right panel) or absence (left panel) of phosphopeptide.
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Western blot - Anti-IFNGR1 (phospho Y457) antibody (ab61062)All lanes : Anti-IFNGR1 (phospho Y457) antibody (ab61062) at 1/500 dilution
Lane 1 : COS7 cell extract with no phosphopeptide
Lane 2 : COS7 cell extract with phosphopeptide
Predicted band size: 54 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
