Factor Xa Assay Kit (ab154128)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Plasma, Serum
- Sensitivity: 0.028 mIU/ml
Overview
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Product name
Factor Xa Assay Kit
See all Factor Xa kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Quantitative -
Sensitivity
0.028 mIU/ml -
Range
0.031 mIU/ml - 2 mIU/ml -
Species reactivity
Reacts with: Human -
Product overview
Factor Xa Assay Kit (ab154128) is developed to determine Factor Xa activity in human plasma, serum and cell culture samples. The assay couples immunofunctional and direct amidolytic function. A polyclonal antibody specific for human Factor Xa has been pre-coated onto a microplate and Factor Xa is bound to the immobilized antibody. The amidolytic activity of the Factor Xa is quantitated by using a highly specific Factor Xa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the Factor Xa enzymatic activity.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Factor X (FX) is a plasma serine protease zymogen involved in the blood coagulation cascade. FX is purified from plasma as a two-chain protein consisting of a 45-kDa heavy chain and a 17-kDa light chain. The FX heavy chain in cleaved during coagulation by several different proteases, including the intrinsic Xase complex, the FX-activating enzyme from Russell's viper venom (RVV) and trypsin, and also by the extrinsic (tissue factor/factor VIIa) pathway, to give an active enzyme FXa. FXa, as the activator of prothrombin, occupies a central position linking the two blood coagulation pathways.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 10X Diluent M Concentrate 1 x 20ml FXa Substrate 2 vials Human FXa Microplate 1 unit Human FXa Standard 1 vial Sealing Tapes 3 units Wash Buffer Concentrate (20x) 1 x 30ml -
Research areas
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Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. -
Tissue specificity
Plasma; synthesized in the liver. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Activated factor Xa heavy chain
- Coagulation factor X
- F10
see all -
Database links
- Entrez Gene: 2159 Human
- Omim: 227600 Human
- SwissProt: P00742 Human
- Unigene: 361463 Human
Images
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Human Factor Xa Chromogenic activity Standard Curve.
