Factor Xa Activity Assay Kit (Fluorometric) (ab204711)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Plasma, Purified protein
- Sensitivity: 1 ng
Overview
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Product name
Factor Xa Activity Assay Kit (Fluorometric)
See all Factor Xa kits -
Detection method
Fluorescent -
Sample type
Plasma, Purified protein -
Assay type
Enzyme activity -
Sensitivity
Assay time
0h 40mProduct overview
Factor Xa Activity Assay Kit (Fluorometric) (ab204711) utilizes the ability of Factor Xa to cleave a synthetic substrate thereby releasing a fluorophore, AMC, which can be quantified by fluorescence readers. This assay kit is simple, rapid and can detect Factor Xa activity as low as 1 ng.
Factor Xa activity assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader for 30-60 min every 2-3 minFor plasma samples, citrate plasma or heparin plasma is recommended as EDTA may interfere with the enzymatic assay.
Notes
Factor Xa (FXa) is the activated form of the coagulation factor X (Stuart-Power factor, thrombokinase, prothrombinase, thromboplastin, E.C.3.4.21.6). Factor X, a serine endopeptidase plays an important role at several stages of the coagulation pathway. It acts by converting prothrombin into active thrombin by complexing with activated co-factor V in the prothrombinase complex. Unfractionated heparin and various low molecular weight heparins bind to plasma cofactor antithrombin to inactivate several coagulation factors including factor Xa.
Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests FXa Assay Buffer 1 x 15ml FXa Dilution Buffer 1 x 1ml FXa Enzyme Standard 1 x 5µl FXa Substrate 1 x 200µl -
Research areas
-
Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. -
Tissue specificity
Plasma; synthesized in the liver. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Activated factor Xa heavy chain
- Coagulation factor X
- F10
see all
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests FXa Assay Buffer 1 x 15ml FXa Dilution Buffer 1 x 1ml FXa Enzyme Standard 1 x 5µl FXa Substrate 1 x 200µl -
Research areas
-
Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. -
Tissue specificity
Plasma; synthesized in the liver. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Activated factor Xa heavy chain
- Coagulation factor X
- F10
see all
Images
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Factor Xa Activity Assay Kit (Fluorometric) (ab204711)
Standard plot of FXa activity measured at two different emission wavelengths (450 and 460 nm) keeping the excitation at 350 nm.
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Factor Xa Activity Assay Kit (Fluorometric) (ab204711)FXa activity was measured in plasma samples in the presence and absence of a FXa inhibitor, GGACK Dihydrochloride. S = Substrate, I = Inhibitor.
