Factor X Human Chromogenic Activity Assay Kit (ab108833)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Plasma
- Sensitivity: 0.125 µg/ml
Overview
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Product name
Factor X Human Chromogenic Activity Assay Kit
See all Factor X kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Plasma -
Assay type
Enzyme activity (quantitative) -
Sensitivity
= 0.125 µg/ml -
Range
0.125 µg/ml - 8 µg/ml -
Species reactivity
Reacts with: Human -
Product overview
Factor X Human Chromogenic Activity Assay Kit (ab108833) is developed to determine human Factor X activity in plasma and cell culture. The assay measures the activation of zymogen Factor X to Factor Xa by RVV. The amidolytic activity of the Factor Xa is quantitated using a highly specific Factor Xa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the Factor X enzymatic activity.
High level of FX activity samples: range of 0.125-8 µg/ml Low level of FX activity samples: range of 0.0156-1 µg/ml
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Factor X is a plasma serine protease zymogen involved in the blood coagulation cascade. Factor X is purified from plasma as a two-chain protein consisting of a 45-kDa heavy chain and a 17-kDa light chain. The Factor X heavy chain is cleaved during coagulation by several different proteases including the intrinsic Xase complex, the Factor X-activating enzyme from Russell’s viper venom (RVV) and trypsin, and also by extrinsic (tissue Factor/Factor VIIa) pathway to give an active enzyme Factor Xa. Factor Xa as the activator of prothrombin occupies a central position linking the two blood coagulation pathways.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Diluent 1 x 5ml Factor X Standard 1 unit Factor Xa Substrate 2 vials Microplate 1 unit RVV 1 vial Sample Diluent 1 x 5ml Sealing Tapes 3 units -
Research areas
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Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. -
Tissue specificity
Plasma; synthesized in the liver. -
Involvement in disease
Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Activated factor Xa heavy chain
- Coagulation factor X
- F10
see all -
Database links
- Entrez Gene: 2159 Human
- Omim: 227600 Human
- SwissProt: P00742 Human
- Unigene: 361463 Human
Images
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Functional Studies - Factor X Human Chromogenic Activity Assay Kit (ab108833)
Factor X Chromogenic Activity measured in cell culture supernatants showing quantity (ug) per mL of tested sample after 40 min of incubation. Values calculated based on Low Level Standard Curve. Samples were diluted 2-10 fold.
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Functional Studies - Factor X Human Chromogenic Activity Assay Kit (ab108833)Factor X Chromogenic Activity measured in biological fluids showing quantity (ug) per mL of tested sample after 10 min of incubation. Values calculated based on High Level Standard Curve. Samples were diluted 2 fold.
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Functional Studies - Factor X Human Chromogenic Activity Assay Kit (ab108833)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Factor X Human Chromogenic Activity Assay Kit (ab108833)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Factor X Human Chromogenic Activity Assay Kit - 1 x 96 wells plate (ab108833)