EDG2 / LPA-1 overexpression 293T lysate (whole cell) (ab94195)
Overview
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Product name
EDG2 / LPA-1 overexpression 293T lysate (whole cell) -
General notes
ab94195 is a 293T cell transfected lysate in which Human EDG2 / LPA-1 has been transiently over-expressed using a pCMV-EDG2 / LPA-1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation. Tissue specificity: Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes. Similarity: Belongs to the G-protein coupled receptor 1 family.
Images
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SDS-PAGE - EDG2 / LPA-1 overexpression 293T lysate (whole cell) (ab94195)ab94195 at 15μg/lane on an SDS-PAGE gel
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Western blot - EDG2 / LPA-1 overexpression 293T lysate (whole cell) (ab94195)All lanes : Anti-EDG2 /LPA-1 antibody (ab89628) at 1/500 dilution
Lane 1 :EDG2 / LPA-1 overexpression 293T lysate (whole cell) (ab94195)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
