D-Lactate Assay Kit (Fluorometric) (ab174096)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Other biological fluids, Plasma, Serum, Suspension cells, Tissue, Urine
- Sensitivity: 0.1 µM
Overview
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Product name
D-Lactate Assay Kit (Fluorometric)
See all D-Lactate kits -
Detection method
Fluorescent -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
>= 0.1 µM -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's PicoProbe D-Lactate Assay Kit (Fluorometric) (ab174096) offers simplicity, sensitivity, and can be adapted to high-throughput research. The assay enzymatically oxidizes D-Lactate generating a fluorescent signal (Ex/Em = 535/587 nm). The signal is directly proportional to the amount of D-Lactate. The assay kit can detect D-Lactate as low as 0.1 µM in a variety of samples.
Visit our FAQs page for tips and troubleshooting.
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Notes
D-Lactate is the result of anaerobic glycolysis by microorganisms in the gastrointestinal system, and the product of detoxification of methylglyoxal by the glyoxalase system. The presence of abnormal levels of D-Lactate has been linked to a series of pathological conditions, such as diabetes, and appendicitis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests D-Lactate Assay Buffer WM 1 x 25ml D-Lactate Enzyme Mix (Lyophilised) Green 1 vial D-Lactate Standard (100 mM) Yellow 1 x 0.1ml D-Lactate Substrate Mix (Lyophilised) Red 1 vial PicoProbe Blue 1 x 0.4ml -
Research areas
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Relevance
D-Lactate production in mammals, mainly due to the glyoxalase pathway, is extremely low, with normal serum concentrations in the nano to micromolar range. Typically, elevated D-lactate levels which can rise to millimolar levels, are due to bacterial infection or short bowel syndrome in humans. Abnormally high concentrations of D-lactate are considered indicative of sepsis, ischemia or trauma. Due to slow metabolism and excretion, high D-lactate can cause acidosis and encephalopathy. -
Alternative names
- D-Lactic acid
Images
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D-Lactate Standard Curve
D-Lactate Standard curve. This assay specifically detected D-Lactate (blue diamonds) but not L-Lactate (red squares). This is example data only.
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Measurement of D-Lactate in urineDiluted samples (1/10, non-diabetic; 1/100 diabetic) were spiked with known amounts of D-Lactate (0-200 pmol) and assayed following kit's protocol. This is example data only.
