Cytohesin 1 overexpression 293T lysate (whole cell) (ab94252)
Overview
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Product name
Cytohesin 1 overexpression 293T lysate (whole cell) -
General notes
ab94252 is a 293T cell transfected lysate in which Human Cytohesin 1 has been transiently over-expressed using a pCMV-Cytohesin 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP. Tissue specificity: Ubiquitous. Similarity: Contains 1 PH domain. Contains 1 SEC7 domain.
Images
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SDS-PAGE - Cytohesin 1 overexpression 293T lysate (whole cell) (ab94252)ab94252 at 15μg/lane on an SDS-PAGE gel
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Western blot - Cytohesin 1 overexpression 293T lysate (whole cell) (ab94252)All lanes : Anti-Cytohesin 1 antibody (ab56511) at 1/500 dilution
Lane 1 :Cytohesin 1 overexpression 293T lysate (whole cell) (ab94252)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
