Bovine IL-10 ELISA Kit (ab277386)
Key features and details
- Sensitivity: 420 pg/ml
- Range: 420 pg/ml - 7000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
Overview
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Product name
Bovine IL-10 ELISA Kit
See all IL-10 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
420 pg/ml -
Range
420 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 130.5 120% - 139% Plasma 110.3 71% - 139% Cell culture media 109.5 79% - 140% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cow -
Product overview
Bovine IL-10 ELISA Kit (ab277386) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine IL-10 serum (bovine IL-10 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Bovine IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and Bovine IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Bovine IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Biotinylated Anti-Bovine IL-10 Antibody 2 vials Anti-Bovine IL-10 coated Microplate 1 unit Bovine IL-10 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.