Bovine Fibronectin ELISA Kit (ab273190)
Key features and details
- Sensitivity: 1.2 ng/ml
- Range: 1.2 ng/ml - 300 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
Overview
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Product name
Bovine Fibronectin ELISA Kit
See all Fibronectin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.2 ng/ml -
Range
1.2 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 120 104% - 140% Plasma 115.1 93% - 133% Cell culture media 110.8 94% - 134% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cow -
Product overview
Bovine Fibronectin ELISA Kit (ab273190) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine Fibronectin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Bovine Fibronectin coated on a 96-well plate. Standards and samples are pipetted into the wells and Fibronectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine Fibronectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fibronectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 1500X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Bovine Fibronectin coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Bovine Fibronectin Antibody 2 vials Bovine Fibronectin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
Tissue specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
Involvement in disease
Glomerulopathy with fibronectin deposits 2 -
Sequence similarities
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
Developmental stage
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
Post-translational
modificationsSulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Alternative names
- CIG
- Cold insoluble globulin
- Cold-insoluble globulin
see all -
Database links
- Entrez Gene: 280794 Cow
- SwissProt: P07589 Cow
Images
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Example standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.