Human Fibronectin ELISA Kit (ab119524)
Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.31 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Fibronectin ELISA Kit
See all Fibronectin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 5.3% Inter-assay Sample n Mean SD CV% Overall 8 6.7% -
Sample type
Cell culture supernatant, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.1 ng/ml -
Range
0.31 ng/ml - 20 ng/ml -
Recovery
110.6 %
Sample specific recovery Sample type Average % Range Serum 110.6 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Fibronectin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human fibronectin concentrations in cell culture supernatant, serum, plasma (EDTA, heparin) and urine.
Fibronectin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an fibronectin biotinylated detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using was buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of fibronectin captured on the plate.
Get results in 90 minutes with Human Fibronectin ELISA Kit (ab181419) from our SimpleStep ELISA® range.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Assay Buffer Concentrate 3 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-human Fibronectin polyclonal antibody 1 x 70µl Fibronectin Standard lyophilized (40 ng/mL upon reconstitution) 2 vials Microplate coated with polyclonal antibody to fibronectin (12 x 8 wells) 1 unit Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 200µl TMB Substrate Solution 1 x 15ml -
Research areas
-
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
Tissue specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
Involvement in disease
Glomerulopathy with fibronectin deposits 2 -
Sequence similarities
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
Developmental stage
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
Post-translational
modificationsSulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
-
Alternative names
- CIG
- Cold insoluble globulin
- Cold-insoluble globulin
see all -
Database links
- Entrez Gene: 2335 Human
- Omim: 135600 Human
- SwissProt: P02751 Human
- Unigene: 203717 Human
Images
-
Typical Standard Curve
Representative Standard Curve using ab119524.
