Bisbenzimide H 33258 (Hoechst 33258), adenine-thymine selective DNA fluorescent stain (ab145596)
Key features and details
- Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent
- CAS Number: 23491-45-4
- Purity: > 98%
- Soluble in water to 10 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Bisbenzimide H 33258 (Hoechst 33258), adenine-thymine selective DNA fluorescent stain -
Description
Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent -
Alternative names
- Hoechst 33258
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Biological description
Cell-permeable, adenine-thymine selective DNA fluorescent stain. DNA minor groove-directed agent. Labels DNA, chromosomes and nuclei. Stimulates topoisomerase I-mediated DNA cleavage. Bisbenzimide H 33342 (ab145596) analog.
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Purity
> 98% -
CAS Number
23491-45-4 -
Chemical structure
Properties
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Excitation
350nm -
Emission
461nm -
Chemical name
4-[5-[6-(4-Methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1,3-dihydrobenzimidazol-2-ylidene]cyclohexa-2,5-dien-1-one trihydrochloride -
Molecular weight
533.88 -
Molecular formula
C25H24N6O.3HCl -
PubChem identifier
5360743 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 10 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)NC(=C6C=CC(=O)C=C6)N5.Cl.Cl.Cl -
Source
Synthetic
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Research areas
