Biotin Anti-Macrophage Inflammatory Protein 1 gamma antibody (ab271205)
Key features and details
- Biotin Rabbit polyclonal to Macrophage Inflammatory Protein 1 gamma
- Suitable for: WB, Sandwich ELISA
- Reacts with: Mouse, Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Overview
-
Product name
Biotin Anti-Macrophage Inflammatory Protein 1 gamma antibody
See all Macrophage Inflammatory Protein 1 gamma primary antibodies -
Description
Biotin Rabbit polyclonal to Macrophage Inflammatory Protein 1 gamma -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA MouseWB Recombinant fragment
-
Immunogen
Recombinant fragment corresponding to Mouse Macrophage Inflammatory Protein 1 gamma aa 1-150.
Database link: P51670 -
Positive control
- WB: Recombinant mouse Macrophage Inflammatory Protein 1 gamma protein.
Properties
-
Form
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Biotin Anti-Macrophage Inflammatory Protein 1 gamma antibody (ab271205)
To detect Murine MIP-1γ by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine MIP-1γ is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse Macrophage Inflammatory Protein 1 gamma, respectively.
Non-reducing conditions.
-
Sandwich ELISA - Biotin Anti-Macrophage Inflammatory Protein 1 gamma antibody (ab271205)Mouse MIP-1γ was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271205.
This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mMIP-1γ protein.