Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD4 antibody [BL-155-1C11] - BSA free (ab243872)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-155-1C11] to CD4 - BSA free
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-CD4 antibody [BL-155-1C11] - BSA free
See all CD4 primary antibodies
Description

Rabbit monoclonal [BL-155-1C11] to CD4 - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human CD4 (extracellular).
Database link: P01730
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-155-1C11
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CD4 antibody [BL-155-1C11] - BSA free (ab243872)

CD4 was immunoprecipitated from 1 mg MOLT-4 whole cell lysate with ab243872 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243872 at 1/1000 dilution.
Lane 1: ab243872 IP in MOLT-4 cell lysate.

Lane 2: Contol IgG in MOLT-4 cell lysate.

Detection: Chemiluminescence with an exposure time of 3 minutes
Western blot - Anti-CD4 antibody [BL-155-1C11] - BSA free (ab243872)

Western blot analysis using ab243872 at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate (15 µg).
Lane 2: MOLT-4 whole cell lysate (15 µg).
Lane3: U-937 whole cell lysate (15 µg).
Lane 4: Ramos whole cell lysate (15 µg).
Lane 5: SR whole cell lysate (15 µg).
Lane 6: HL-60 whole cell lysate (15 µg).
Lane 7: HeLa whole cell lysate (15µg).
Lane 8:HEK-293T whole cell lysate (15 µg).
Lane 9: HEPG2 whole cell lysate (15 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [BL-155-1C11] - BSA free (ab243872)

Formalin-fixed, paraffin-embedded human appendix tissue stained for CD4 using ab243872 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Anti-CD4 antibody [BL-155-1C11] - BSA free (ab243872)

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