Biotin Anti-IL-4 antibody (ab84278)
Key features and details
- Biotin Rabbit polyclonal to IL-4
- Suitable for: Sandwich ELISA, WB
- Reacts with: Rat
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-IL-4 antibody
See all IL-4 primary antibodies -
Description
Biotin Rabbit polyclonal to IL-4 -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA RatWB Rat
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Immunogen
Recombinant full length protein corresponding to Rat IL-4.
Properties
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Form
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purification notes
ab84278 was purified by affinity chromatography and then biotinylated. It is sterile filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Biotin Anti-IL-4 antibody (ab84278)
To detect rat IL-4 by Western Blot analysis ab84278 can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Rat recombinant IL-4 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 rat recombinant IL-4 protein.
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Sandwich ELISA - Biotin Anti-IL-4 antibody (ab84278)To detect rat IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84278 is required. Using this antibody in conjunction with an anti-IL-4 (ab9811) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant rat IL-4.
