Biotin Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110430)
![Biotin Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110430)](https://www.abcam.com/ps/products/110/ab110430/Images/ab110430-199657-anti-dmpo-nitrone-adduct-antibody-n1664a-biotin-immunofluorescence.jpg "Biotin Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110430)")
Key features and details
- Biotin Mouse monoclonal [N1664A] to DMPO Nitrone Adduct
- Suitable for: IP, ICC/IF, WB
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-DMPO Nitrone Adduct antibody [N1664A]
See all DMPO Nitrone Adduct primary antibodies -
Description
Biotin Mouse monoclonal [N1664A] to DMPO Nitrone Adduct -
Host species
Mouse -
Conjugation
Biotin -
Specificity
Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA. -
Tested applications
Suitable for: IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to DMPO Nitrone Adduct conjugated to ovalbumin. 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.
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Positive control
- ICC/IF: RAW 264.7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N1664A -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Biotin Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110430)
RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for DMPO Nitrone Adduct using the unconjugated version of ab110430 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Mouse IgG (Green). Counterstain: DAPI (Blue) nuclear stain.
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![Western blot - Biotin Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110430)](https://www.abcam.com/ps/products/110/ab110430/Images/ab110430-199656-anti-dmpo-nitrone-adduct-antibody-n1664a-biotin-western-blot.jpg)
Western blot - Biotin Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110430)All lanes : Unconjugated version of ab110430 at 1/200 dilution
Lane 1 : 10 um hemoglobin
Lane 2 : 10 um hemoglobin + 100 uM HOCl
Lane 3 : 10 um hemoglobin + 500 uM HOCl
Lane 4 : 10 um hemoglobin + 1000 uM HOCl
Lane 5 : 10 um hemoglobin + 20 mM DMPO
Lane 6 : 10 um hemoglobin + 100 uM HOCl + 20 mM DMPO
Lane 7 : 10 um hemoglobin + 500 uM HOCl + 20 mM DMPO
Lane 8 : 10 um hemoglobin + 1000 uM HOCl + 20 mM DMPO
Lane 9 : 10 um hemoglobin + 100 mM DMPO
Lane 10 : 10 um hemoglobin + 100 uM HOCl + 100 mM DMPO
Lane 11 : 10 um hemoglobin + 500 uM HOCl + 100 mM DMPO
Lane 12 : 10 um hemoglobin + 1000 uM HOCl + 100 mM DMPO
