Antibodies, Proteins, Kits and Reagents for Life Science

Biotin Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110430)

Key features and details

Biotin Mouse monoclonal [N1664A] to DMPO Nitrone Adduct
Suitable for: IP, ICC/IF, WB
Reacts with: Species independent
Conjugation: Biotin
Isotype: IgG1

Product name

Biotin Anti-DMPO Nitrone Adduct antibody [N1664A]
See all DMPO Nitrone Adduct primary antibodies
Description

Biotin Mouse monoclonal [N1664A] to DMPO Nitrone Adduct
Host species

Mouse
Conjugation

Biotin
Specificity

Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA.
Tested applications

Suitable for: IP, ICC/IF, WBmore details
Species reactivity

Reacts with: Species independent
Immunogen

Chemical/ Small Molecule corresponding to DMPO Nitrone Adduct conjugated to ovalbumin. 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.
Positive control
- ICC/IF: RAW 264.7 cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

N1664A
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Biotin Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110430)

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for DMPO Nitrone Adduct using the unconjugated version of ab110430 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Mouse IgG (Green). Counterstain: DAPI (Blue) nuclear stain.
Western blot - Biotin Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110430)

All lanes : Unconjugated version of ab110430 at 1/200 dilution

Lane 1 : 10 um hemoglobin
Lane 2 : 10 um hemoglobin + 100 uM HOCl
Lane 3 : 10 um hemoglobin + 500 uM HOCl
Lane 4 : 10 um hemoglobin + 1000 uM HOCl
Lane 5 : 10 um hemoglobin + 20 mM DMPO
Lane 6 : 10 um hemoglobin + 100 uM HOCl + 20 mM DMPO
Lane 7 : 10 um hemoglobin + 500 uM HOCl + 20 mM DMPO
Lane 8 : 10 um hemoglobin + 1000 uM HOCl + 20 mM DMPO
Lane 9 : 10 um hemoglobin + 100 mM DMPO
Lane 10 : 10 um hemoglobin + 100 uM HOCl + 100 mM DMPO
Lane 11 : 10 um hemoglobin + 500 uM HOCl + 100 mM DMPO
Lane 12 : 10 um hemoglobin + 1000 uM HOCl + 100 mM DMPO

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