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Signal Transduction Metabolism Mitochondrial

Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)

Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [N1664A] to DMPO Nitrone Adduct
  • Suitable for: WB, ELISA, ICC/IF, IP
  • Reacts with: Species independent
  • Isotype: IgG1

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Overview

  • Product name

    Anti-DMPO Nitrone Adduct antibody [N1664A]
    See all DMPO Nitrone Adduct primary antibodies
  • Description

    Mouse monoclonal [N1664A] to DMPO Nitrone Adduct
  • Host species

    Mouse
  • Specificity

    Detects ~90kDa. Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA.
  • Tested applications

    Suitable for: WB, ELISA, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), Tris buffered saline
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N1664A
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)
    Immunocytochemistry/ Immunofluorescence - Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)

    Immuncytochemistry/Immunofluorescence analysis of Mouse macrophage cells labelling DMPO Nitrone Adduct with ab104902 at 10μg/mL. Left - with DMPO, Right - no DMPO. Top to bottom - DMPO, DAPI, overlay.

  • Western blot - Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)
    Western blot - Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)
    All lanes : Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902) at 10 µg/ml

    Lane 1 : 10µM Hb
    Lane 2 : 10µM Hb + 100µM HOCL
    Lane 3 : 10µM Hb + 500µM HOCL
    Lane 4 : 10µM Hb + 1000µM HOCL
    Lane 5 : 10µM Hb with 20mM DMPO
    Lane 6 : 10µM Hb + 100µM HOCL with 20mM DMPO
    Lane 7 : 10µM Hb + 500µM HOCL with 20mM DMPO
    Lane 8 : 10µM Hb + 1000µM HOCL with 20mM DMPO
    Lane 9 : 10µM Hb with 100mM DMPO
    Lane 10 : 10µM Hb + 100µM HOCL with 100mM DMPO
    Lane 11 : 10µM Hb + 500µM HOCL with 100mM DMPO
    Lane 12 : 10µM Hb + 1000µM HOCL with 100mM DMPO

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: ICC

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    Applications: ELISA, WB

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    Biotin Anti-DMPO Nitrone Adduct antibody [N1664A] (ab110430)

    Applications: ICC/IF, IP, WB

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