Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902)
Key features and details
- Mouse monoclonal [N1664A] to DMPO Nitrone Adduct
- Suitable for: WB, ELISA, ICC/IF, IP
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-DMPO Nitrone Adduct antibody [N1664A]
See all DMPO Nitrone Adduct primary antibodies -
Description
Mouse monoclonal [N1664A] to DMPO Nitrone Adduct -
Host species
Mouse -
Specificity
Detects ~90kDa. Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA. -
Tested applications
Suitable for: WB, ELISA, ICC/IF, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), Tris buffered saline -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N1664A -
Isotype
IgG1 -
Research areas
Images
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Immuncytochemistry/Immunofluorescence analysis of Mouse macrophage cells labelling DMPO Nitrone Adduct with ab104902 at 10μg/mL. Left - with DMPO, Right - no DMPO. Top to bottom - DMPO, DAPI, overlay.
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All lanes : Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902) at 10 µg/ml
Lane 1 : 10µM Hb
Lane 2 : 10µM Hb + 100µM HOCL
Lane 3 : 10µM Hb + 500µM HOCL
Lane 4 : 10µM Hb + 1000µM HOCL
Lane 5 : 10µM Hb with 20mM DMPO
Lane 6 : 10µM Hb + 100µM HOCL with 20mM DMPO
Lane 7 : 10µM Hb + 500µM HOCL with 20mM DMPO
Lane 8 : 10µM Hb + 1000µM HOCL with 20mM DMPO
Lane 9 : 10µM Hb with 100mM DMPO
Lane 10 : 10µM Hb + 100µM HOCL with 100mM DMPO
Lane 11 : 10µM Hb + 500µM HOCL with 100mM DMPO
Lane 12 : 10µM Hb + 1000µM HOCL with 100mM DMPO