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APC Anti-Integrin alpha 2 antibody [DX5] (ab41460)

Key features and details

  • APC Rat monoclonal [DX5] to Integrin alpha 2
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Conjugation: APC. Ex: 645nm, Em: 660nm
  • Isotype: IgM

Overview

  • Product name

    APC Anti-Integrin alpha 2 antibody [DX5]
    See all Integrin alpha 2 primary antibodies

  • Description

    APC Rat monoclonal [DX5] to Integrin alpha 2

  • Host species

    Rat

  • Conjugation

    APC. Ex: 645nm, Em: 660nm

  • Specificity

    Mouse Integrin alpha 2 (CD49b), 160kDa

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    See all applications and species data

  • Immunogen

    RIL-2-propagated NK1.1+ cells from C57BL/6 mice

  • Positive control

    • Flow Cyt: C57BL/6 mouse splenocytes.

  • General notes

    This monoclonal antibody, clone DX5, reacts with CD49b, also known as very late antigen-2 (VLA-2) and Integrin alpha 2. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.3
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 16% Sucrose

    Also contains stabilising agent.
  • Concentration information loading...

  • Purity

    Purified IgM

  • Primary antibody notes

    This monoclonal antibody, clone DX5, reacts with CD49b, also known as very late antigen-2 (VLA-2) and Integrin alpha 2. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity.

  • Clonality

    Monoclonal

  • Clone number

    DX5

  • Myeloma

    Sp2/0

  • Isotype

    IgM

  • Light chain type

    kappa

  • Research areas

Images

  • Flow Cytometry - APC Anti-Integrin alpha 2 antibody [DX5] (ab41460)
    Flow Cytometry - APC Anti-Integrin alpha 2 antibody [DX5] (ab41460)

    Flow Cytometry analysis of C57BL/6 mouse splenocytes stained for Integrin alpha 2 using ab41460 (0.1 µg/106 cells) and a Mouse Anti-Mouse NK1.1-PE.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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