Anti-Integrin alpha 2 antibody [EPR5789] (ab109432)
![Anti-Integrin alpha 2 antibody [EPR5789] (ab109432)](https://www.abcam.com/ps/products/109/ab109432/Images/ab109432-388725-anti-integrin-alpha-2-antibody-epr5789-western-blot-hela-a431-k562-lysates.jpg "Anti-Integrin alpha 2 antibody [EPR5789] (ab109432)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5789] to Integrin alpha 2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Integrin alpha 2 antibody [EPR5789]
See all Integrin alpha 2 primary antibodies -
Description
Rabbit monoclonal [EPR5789] to Integrin alpha 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HAP1, 293T, T47-D, and A431 cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5789 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (ab109432)All lanes : Anti-Integrin alpha 2 antibody [EPR5789] (ab109432) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ITGA2 knockout HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 129 kDa
Observed band size: 129 kDaLanes 1-4: Merged signal (red and green). Green - ab109432 observed at 129 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109432 Anti-Integrin alpha 2 antibody [EPR5789] was shown to specifically react with Integrin alpha 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264819 (knockout cell lysate ab257262) was used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab109432 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (ab109432)](https://www.abcam.com/ps/products/109/ab109432/Images/ab109432-254637-anti-integrin-alpha-2-antibody-epr5789-western-blot.jpg)
Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (ab109432)All lanes : Anti-Integrin alpha 2 antibody [EPR5789] (ab109432) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : Integrin alpha 2 knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : T47D cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 129 kDaLanes 1 - 4: Merged signal (red and green). Green - ab109432 observed at 165 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109432 was shown to specifically react with Integrin alpha 2 when Integrin alpha 2 knockout samples were used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab109432 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (ab109432)](https://www.abcam.com/ps/products/109/ab109432/Images/ab109432-122268-anti-integrin-alpha-2-antibody-epr5789-western-blot.jpg)
Western blot - Anti-Integrin alpha 2 antibody [EPR5789] (ab109432)All lanes : Anti-Integrin alpha 2 antibody [EPR5789] (ab109432) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : T47-D cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 129 kDa
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![Anti-Integrin alpha 2 antibody [EPR5789] (ab109432)](https://www.abcam.com/ps/products/109/ab109432/Images/ab109432-4-benefits-of-recombinant-antibodies.png)
Anti-Integrin alpha 2 antibody [EPR5789] (ab109432)
