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Neuroscience Neurology process Neurogenesis

Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (ab172612)

Price and availability

284 784 ₸

Availability

Order now and get it on Monday March 15, 2021

Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (ab172612)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4920(2)] to Wnt3 and Wnt3a
  • Suitable for: WB
  • Reacts with: Recombinant fragment

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Overview

  • Product name

    Anti-Wnt3 and Wnt3a antibody [EPR4920(2)]
    See all Wnt3 and Wnt3a primary antibodies
  • Description

    Rabbit monoclonal [EPR4920(2)] to Wnt3 and Wnt3a
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for both Wnt3 and Wnt3a based on the fact that the immunogen sequence is 100% identical for both.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Wnt3 and Wnt3a aa 150-250 (Cysteine residue). The exact sequence is proprietary. SwissProt: P56703 and P56704
    Database link: P56704

  • Positive control

    • CHO cells transfected with Human Wnt3a.
  • General notes

    This antibody was developed as part of a collaboration with Karl Willert’s lab from the University of California at San Diego.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4920(2)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Secreted
    • Developmental Biology
    • Embryogenesis
    • Morphogens
    • Developmental Biology
    • Organogenesis
    • Nervous system development

Images

  • Western blot - Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (ab172612)
    Western blot - Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (ab172612)
    All lanes : Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (ab172612) at 1/10000 dilution

    Lane 1 : CHO cell lysate, non-transfected
    Lane 2 : CHO cell lysate, transfected with Human Wnt3a

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 39 kDa

  • Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (ab172612)
    Anti-Wnt3 and Wnt3a antibody [EPR4920(2)] (ab172612)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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