Anti-LIM1/LHX1 antibody (ab229474)
Key features and details
- Rabbit polyclonal to LIM1/LHX1
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-LIM1/LHX1 antibody
See all LIM1/LHX1 primary antibodies -
Description
Rabbit polyclonal to LIM1/LHX1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Human, Chimpanzee
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Immunogen
Recombinant fragment within Human LIM1/LHX1 (internal sequence). The exact sequence is proprietary.
Database link: P48742 -
Positive control
- IHC-Fr: E13.5 rat brain tissue; Adult mouse retina tissue. WB: Mouse and rat brain tissue extracts. IHC-P: Rat brain tissue; Mouse hind brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)
Paraffin-embedded rat brain tissue stained for LIM1/LHX1 using ab229474 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)Frozen sectioned E13.5 rat brain tissue stained for LIM1/LHX1 using ab229474 at 1/250 dilution in immunohistochemical analysis.
Red: SOX2, stained by SOX2 antibody at 1/250. dilution
Blue: Fluoroshield with DAPI. -
Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)Frozen sectioned adult mouse retina tissue stained for LIM1/LHX1 using ab229474 at 1/250 dilution in immunohistochemical analysis.
Red: Protein kinase C alpha staining.
Blue: Fluorshield with DAPI. -
Western blot - Anti-LIM1/LHX1 antibody (ab229474)All lanes : Anti-LIM1/LHX1 antibody (ab229474) at 1/2000 dilution
Lane 1 : Mouse brain extract
Lane 2 : Rat brain extract
Lysates/proteins at 50 µg per lane.
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)Paraffin-embedded mouse hind brain tissue stained for LIM1/LHX1 using ab229474 at 1/500 dilution in immunohistochemical analysis.
