Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)

Key features and details

  • Mouse monoclonal [10D6] to HCV subtype 1b NS5B
  • Suitable for: WB, IP, ELISA
  • Reacts with: Hepatitis C virus
  • Isotype: IgG1

Overview

  • Product name

    Anti-HCV subtype 1b NS5B antibody [10D6]
    See all HCV subtype 1b NS5B primary antibodies

  • Description

    Mouse monoclonal [10D6] to HCV subtype 1b NS5B

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IP, ELISAmore details

  • Species reactivity

    Reacts with: Hepatitis C virus

  • Immunogen

    Recombinant fragment:

    LSSKAVNHIRSVWKDLLEDT

    , corresponding to amino acids 111-130 of HCV subtype 1b NS5B
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Huh7 cells harboring selectable subgenomic HCV RNA replicon

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Immunoprecipitation - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
    Immunoprecipitation - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
    IP was carried out with ab100895 using the lysates of Huh7 cells harboring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (-NS5B) and -Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with ab100895.
  • Western blot - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
    Western blot - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
    All lanes : Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)

    Lane 1 : Huh-7
    transfected with HCV replicon
    Lane 2 : Huh-7 nontransfected
    cells

    Predicted band size: 65 kDa

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