Anti-LIM1/LHX1 antibody (ab84789)
Key features and details
- Rabbit polyclonal to LIM1/LHX1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LIM1/LHX1 antibody
See all LIM1/LHX1 primary antibodies -
Description
Rabbit polyclonal to LIM1/LHX1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human LIM1/LHX1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab104205) -
Positive control
- This antibody gave a positive signal in Human thymus tissue lysate.
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General notes
This product was previously labelled as LIM1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatRabbitHamsterDogChimpanzeeApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).Target
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Function
Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis. -
Tissue specificity
Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines. -
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
Domain
The LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 454600 Chimpanzee
- Entrez Gene: 3975 Human
- Entrez Gene: 16869 Mouse
- Entrez Gene: 257634 Rat
- Omim: 601999 Human
- SwissProt: Q5IS44 Chimpanzee
- SwissProt: P48742 Human
- SwissProt: P63006 Mouse
see all -
Alternative names
- hLim-1 antibody
- Homeo box protein Lim 1 antibody
- Homeo box protein Lim1 antibody
see all
Images
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Western blot - Anti-LIM1/LHX1 antibody (ab84789)Anti-LIM1/LHX1 antibody (ab84789) at 1 µg/ml + Human thymus tissue lysate - total protein (ab30146) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
Datasheets and documents
References (0)
ab84789 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-LIM1/LHX1 antibody (ab84789)Anti-LIM1/LHX1 antibody (ab84789) at 1 µg/ml + Human thymus tissue lysate - total protein (ab30146) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
