Human DLL1 ELISA Kit (ab193698)
Key features and details
- Sensitivity: 45 pg/ml
- Range: 45 pg/ml - 12000 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich
- Reacts with: Human
Overview
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Product name
Human DLL1 ELISA Kit
See all DLL1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich -
Sensitivity
45 pg/ml -
Range
45 pg/ml - 12000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 100.2 92% - 108% Plasma 87.96 74% - 96% Cell culture media 90.16 82% - 97% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam's DLL1 Human ELISA Kit (ab193698) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DLL1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for human DLL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures DLL1 present in the samples. The wells are washed and biotinylated anti-human DLL1 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of DLL1 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated Human DLL-1 detection antibody (lyophilized) 2 vials Human DLL-1 standards (lyophilized) 2 vials Pre-coated Human DLL-1 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. -
Tissue specificity
Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. -
Sequence similarities
Contains 1 DSL domain.
Contains 8 EGF-like domains. -
Post-translational
modificationsUbiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Delta
- Delta (Drosophila) like 1
- Delta like 1
see all -
Database links
- Entrez Gene: 28514 Human
- Omim: 606582 Human
- SwissProt: O00548 Human
- Unigene: 379912 Human
Images
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Example Standard Curve
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Example Standard Curve