Anti-Wnt10a antibody (ab106522)
Key features and details
- Rabbit polyclonal to Wnt10a
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt10a antibody
See all Wnt10a primary antibodies -
Description
Rabbit polyclonal to Wnt10a -
Host species
Rabbit -
Specificity
Despite the high homology of Wnt10a to Wnt10b, ab106522 will not cross-react with Wnt10b. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A 14 amino acid synthetic peptide from near the C terminus of Human Wnt10a (UniProt Q9GZT5).
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Positive control
- WB: RAW264.7 cell lysate. IHC-P: Human and mouse skeletal muscle tissue; Rat bladder tissue. ICC/IF: Human skeletal muscle cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Lane 1 : Anti-Wnt10a antibody (ab106522) at 1 µg/ml
Lane 2 : Anti-Wnt10a antibody (ab106522) at 2 µg/ml
All lanes : RAW264.7 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 46 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10a antibody (ab106522)
Paraffin-embedded mouse skeletal muscle tissue stained for Wnt10a using ab106522 at 5 µg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10a antibody (ab106522)
Paraffin-embedded human skeletal muscle tissue stained for Wnt10a using ab106522 at 10 µg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10a antibody (ab106522) This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of rat bladder tissue, staining Wnt10a with ab106522.
Tissue was fixed with formalin and blocked with 5000 µg/ml BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An AlexaFluor®555-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody. -
Immunofluorescence of Wnt10a in Human Skeletal Muscle cells using ab106522 at 20 ug/ml.