Anti-CD52 antibody [EPR23855-41] - BSA and Azide free (ab275257)
![Anti-CD52 antibody [EPR23855-41] - BSA and Azide free (ab275257)](https://www.abcam.com/ps/products/275/ab275257/Images/ab275257-1-anti-cd52-antibody-epr2385541-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg "Anti-CD52 antibody [EPR23855-41] - BSA and Azide free (ab275257)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23855-41] to CD52 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD52 antibody [EPR23855-41] - BSA and Azide free
See all CD52 primary antibodies -
Description
Rabbit monoclonal [EPR23855-41] to CD52 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: Human PBMCs.
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General notes
ab275257 is the carrier-free version of ab259794. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275257 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23855-41 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CD52 antibody [EPR23855-41] - BSA and Azide free (ab275257)
This data was developed using ab259794, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD52 with ab259794 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control.
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or ab259794. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.
Gated on viable cells.
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![Anti-CD52 antibody [EPR23855-41] - BSA and Azide free (ab275257)](https://www.abcam.com/ps/products/275/ab275257/Images/ab275257-2-benefits-of-recombinant-antibodies.png)
Anti-CD52 antibody [EPR23855-41] - BSA and Azide free (ab275257)