Anti-VEGFA antibody [16F1] (ab155944)
Key features and details
- Mouse monoclonal [16F1] to VEGFA
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-VEGFA antibody [16F1]
See all VEGFA primary antibodies -
Description
Mouse monoclonal [16F1] to VEGFA -
Host species
Mouse -
Tested applications
Suitable for: WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length Human VEGFA expressed in E-coli (P15692)
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Positive control
- Human VEGFA recombinant protein; Human VEGFA transfected 293T cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
16F1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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All lanes : Anti-VEGFA antibody [16F1] (ab155944) at 1 µg/ml
Lane 1 : VEGFA transfected 293T cell culture supernatant
Lane 2 : Recombinant VEGFA protein
Lysates/proteins at 0.5 µg per lane.
4-12% HCl -
The plate was coated with 100 μL ab155944 at 1, 3, 4, 5 7 and 9 μg/mL in DPBS for 12 - 18 hours at 4°C. After washing the plate was blocked with 300 μL per well of 4% BSA / 5% sucrose in DPBS for 1 hour at room temperature.
Recombinant human VEGF protein (50 μL /well) was added at 2000, 1000, 500, 250, 125, 62.5, 31.25, and 0 pg/mL for 2 hours at room temperature.
After washing the plate was incubated with ab155944 at 0.5 μg /Ml for 1 hour at room temperature.
Detection was with Streptavidin-HRP / TMB.