Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP5 antibody [EPR10454] (ab154170)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10454] to USP5
  • Suitable for: WB, Flow Cyt, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-USP5 antibody [EPR10454]
    See all USP5 primary antibodies

  • Description

    Rabbit monoclonal [EPR10454] to USP5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human USP5. The exact sequence is proprietary.
    Database link: P45974

  • Positive control

    • WB: MCF7 and A549 whole cell lysates. Human, mouse, and rat brain tissue lysates. Mouse and rat heart and kidney tissue lysates; ICC: MCF7 and A549 cells; Flow Cyt: MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-USP5 antibody [EPR10454] (ab154170)
    Western blot - Anti-USP5 antibody [EPR10454] (ab154170)
    All lanes : Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution (purified)

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 3 : Human brain lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Mouse heart lysate
    Lane 7 : Rat heart lysate
    Lane 8 : Mouse kidney lysate
    Lane 9 : Rat kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 96 kDa
    Observed band size: 96 kDa

  • Immunocytochemistry - Anti-USP5 antibody [EPR10454] (ab154170)
    Immunocytochemistry - Anti-USP5 antibody [EPR10454] (ab154170)

    Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling USP5 with purified ab154170 at 1/50 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-USP5 antibody [EPR10454] (ab154170)
    Flow Cytometry - Anti-USP5 antibody [EPR10454] (ab154170)

    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling USP5 with purified ab154170 at 1/50 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-USP5 antibody [EPR10454] (ab154170)
    Western blot - Anti-USP5 antibody [EPR10454] (ab154170)
    All lanes : Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 96 kDa

  • Western blot - Anti-USP5 antibody [EPR10454] (ab154170)
    Western blot - Anti-USP5 antibody [EPR10454] (ab154170)
    All lanes : Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution (unpurified)

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates
    Lane 3 : Rat heart lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 96 kDa

  • Immunocytochemistry - Anti-USP5 antibody [EPR10454] (ab154170)
    Immunocytochemistry - Anti-USP5 antibody [EPR10454] (ab154170)

    Immunofluorescent analysis of MCF7 cells labeling USP5 with unpurified ab154170 at 1/50 dilution.

  • Anti-USP5 antibody [EPR10454] (ab154170)
    Anti-USP5 antibody [EPR10454] (ab154170)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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