Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-USP5 antibody [EPR10454] (ab154170)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10454] to USP5
Suitable for: WB, Flow Cyt, ICC
Reacts with: Mouse, Rat, Human

Product name

Anti-USP5 antibody [EPR10454]
See all USP5 primary antibodies
Description

Rabbit monoclonal [EPR10454] to USP5
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human USP5. The exact sequence is proprietary.
Database link: P45974
Positive control
- WB: MCF7 and A549 whole cell lysates. Human, mouse, and rat brain tissue lysates. Mouse and rat heart and kidney tissue lysates; ICC: MCF7 and A549 cells; Flow Cyt: MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10454
Isotype

IgG
Research areas

Western blot - Anti-USP5 antibody [EPR10454] (ab154170)

All lanes : Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution (purified)

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 3 : Human brain lysate
Lane 4 : Mouse brain lysate
Lane 5 : Rat brain lysate
Lane 6 : Mouse heart lysate
Lane 7 : Rat heart lysate
Lane 8 : Mouse kidney lysate
Lane 9 : Rat kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 96 kDa
Observed band size: 96 kDa
Immunocytochemistry - Anti-USP5 antibody [EPR10454] (ab154170)

Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling USP5 with purified ab154170 at 1/50 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-USP5 antibody [EPR10454] (ab154170)

Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling USP5 with purified ab154170 at 1/50 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-USP5 antibody [EPR10454] (ab154170)

All lanes : Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution (unpurified)

Lane 1 : MCF7 cell lysate
Lane 2 : A549 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 96 kDa
Western blot - Anti-USP5 antibody [EPR10454] (ab154170)

All lanes : Anti-USP5 antibody [EPR10454] (ab154170) at 1/1000 dilution (unpurified)

Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lane 3 : Rat heart lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 96 kDa
Immunocytochemistry - Anti-USP5 antibody [EPR10454] (ab154170)

Immunofluorescent analysis of MCF7 cells labeling USP5 with unpurified ab154170 at 1/50 dilution.
Anti-USP5 antibody [EPR10454] (ab154170)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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