Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-U2AF1L4 antibody [EPR14349] (ab188582)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14349] to U2AF1L4
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-U2AF1L4 antibody [EPR14349]
    See all U2AF1L4 primary antibodies

  • Description

    Rabbit monoclonal [EPR14349] to U2AF1L4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, Human cerebellum, K562, Mouse brain and PC-12 lysates; U87-MG cells.

  • General notes

     

     

     This product was previously labelled as U2AF1L3

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Flow Cytometry - Anti-U2AF1L4 antibody [EPR14349] (ab188582)
    Flow Cytometry - Anti-U2AF1L4 antibody [EPR14349] (ab188582)

    ab188582 staining U2AF1L4 in A549 (human lung carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Immunocytochemistry/ Immunofluorescence - Anti-U2AF1L4 antibody [EPR14349] (ab188582)
    Immunocytochemistry/ Immunofluorescence - Anti-U2AF1L4 antibody [EPR14349] (ab188582)

    Immunofluorescent analysis of paraformaldehyde-fixed U87-MG cells labeling U2AF1L4 with ab188582 at 1/250 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI staining.

  • Western blot - Anti-U2AF1L4 antibody [EPR14349] (ab188582)
    Western blot - Anti-U2AF1L4 antibody [EPR14349] (ab188582)
    All lanes : Anti-U2AF1L4 antibody [EPR14349] (ab188582)

    Lane 1 : Mouse brain lysate
    Lane 2 : PC-12 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa
    Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-U2AF1L4 antibody [EPR14349] (ab188582)
    Western blot - Anti-U2AF1L4 antibody [EPR14349] (ab188582)
    All lanes : Anti-U2AF1L4 antibody [EPR14349] (ab188582) at 1/10000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human cerebellum lysate
    Lane 3 : K562 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa
    Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.

  • Anti-U2AF1L4 antibody [EPR14349] (ab188582)
    Anti-U2AF1L4 antibody [EPR14349] (ab188582)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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