Antibodies, Proteins, Kits and Reagents for Life Science

264 681 ₸ Product size

100 µl 264 681 ₸

Order now and get it on Tuesday March 09, 2021

Anti-U2AF1L4 antibody [EPR14349] (ab188582)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14349] to U2AF1L4
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-U2AF1L4 antibody [EPR14349]
See all U2AF1L4 primary antibodies
Description

Rabbit monoclonal [EPR14349] to U2AF1L4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain, Human cerebellum, K562, Mouse brain and PC-12 lysates; U87-MG cells.
General notes
This product was previously labelled as U2AF1L3

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14349
Isotype

IgG
Research areas

Flow Cytometry - Anti-U2AF1L4 antibody [EPR14349] (ab188582)

ab188582 staining U2AF1L4 in A549 (human lung carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control: Rabbit monoclonal IgG (Black)

Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Immunocytochemistry/ Immunofluorescence - Anti-U2AF1L4 antibody [EPR14349] (ab188582)

Immunofluorescent analysis of paraformaldehyde-fixed U87-MG cells labeling U2AF1L4 with ab188582 at 1/250 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI staining.
Western blot - Anti-U2AF1L4 antibody [EPR14349] (ab188582)

All lanes : Anti-U2AF1L4 antibody [EPR14349] (ab188582)

Lane 1 : Mouse brain lysate
Lane 2 : PC-12 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-U2AF1L4 antibody [EPR14349] (ab188582)

All lanes : Anti-U2AF1L4 antibody [EPR14349] (ab188582) at 1/10000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human cerebellum lysate
Lane 3 : K562 lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.
Anti-U2AF1L4 antibody [EPR14349] (ab188582)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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