Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (ab255743)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [2N23] to Uroplakin Ib/UPIb - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free
    See all Uroplakin Ib/UPIb primary antibodies

  • Description

    Mouse monoclonal [2N23] to Uroplakin Ib/UPIb - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse and rat bladder tissue. Rat kidney tissue. IHC-Fr: Mouse and rat kidney tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255743 is the carrier-free version of ab237777. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    2N23

  • Isotype

    IgG2b

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (ab255743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (ab255743)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat kidney tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab237777).

  • Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (ab255743)
    Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (ab255743)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 3.94µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution (Green). Positive staining on rat kidney is observed. Nuclear counterstain is DAPI (Blue).
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab237777).

  • Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (ab255743)
    Immunohistochemistry (Frozen sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (ab255743)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Uroplakin Ib/UPIb with ab237777 at 3.94µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution (Green). Positive staining on mouse kidney is observed. Nuclear counterstain is DAPI (Blue).
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at a 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab237777).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (ab255743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (ab255743)

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab237777).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (ab255743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (ab255743)

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue labeling Uroplakin Ib/UPIb with ab237777 at 0.099µg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse bladder tissue is observed. The section was incubated with ab237777 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab237777).

  • Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (ab255743)
    Anti-Uroplakin Ib/UPIb antibody [2N23] - BSA and Azide free (ab255743)

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