Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLC27A4 / FATP4 antibody [EPR17319] - Low endotoxin, Azide free (ab221783)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17319] to SLC27A4 / FATP4 - Low endotoxin, Azide free
  • Suitable for: IHC-P, IP, ICC/IF, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SLC27A4 / FATP4 antibody [EPR17319] - Low endotoxin, Azide free
    See all SLC27A4 / FATP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR17319] to SLC27A4 / FATP4 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK293 and HepG2 whole cell lysates; Human fetal brain and Human fetal kidney lysates. IHC-P: Human kidney tissue. ICC/IF: HepG2 cells. IP: HEK293 whole cell lysate.

  • General notes

    ab221783 is the carrier-free version of ab199719.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Properties

Images

  • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - Low endotoxin, Azide free (ab221783)
    Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - Low endotoxin, Azide free (ab221783)
    All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : SLC27A4 knockout HEK293T cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HEK-293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab199719).

    Lanes 1-4: Merged signal (red and green). Green - ab199719 observed at 72 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab199719 Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal  was shown to specifically react with SLC27A4 / FATP4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266114 (knockout cell lysate ab257677) was used. Wild-type and SLC27A4 / FATP4 knockout samples were subjected to SDS-PAGE. ab199719 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-SLC27A4 / FATP4 antibody [EPR17319] - Low endotoxin, Azide free (ab221783)
    Immunocytochemistry/ Immunofluorescence - Anti-SLC27A4 / FATP4 antibody [EPR17319] - Low endotoxin, Azide free (ab221783)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling SLC27A4 / FATP4 with ab199719 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HepG2 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1: ab199719 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199719).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A4 / FATP4 antibody [EPR17319] - Low endotoxin, Azide free (ab221783)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A4 / FATP4 antibody [EPR17319] - Low endotoxin, Azide free (ab221783)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SLC27A4 / FATP4 with ab199719 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199719).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-SLC27A4 / FATP4 antibody [EPR17319] - Low endotoxin, Azide free (ab221783)
    Immunoprecipitation - Anti-SLC27A4 / FATP4 antibody [EPR17319] - Low endotoxin, Azide free (ab221783)

    SLC27A4 / FATP4 was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab199719 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab199719 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HEK293  whole cell lysate 10µg (Input). Lane 2: HEK293 whole cell lysate following IP. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199719 in HEK293 whole cell lysate.


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure is 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199719).

  • Anti-SLC27A4 / FATP4 antibody [EPR17319] - Low endotoxin, Azide free (ab221783)
    Anti-SLC27A4 / FATP4 antibody [EPR17319] - Low endotoxin, Azide free (ab221783)

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