Human Attractin ELISA Kit (ab263888)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 ng/ml
- Range: 2.81 ng/ml - 180 ng/ml
- Sample type: Cell culture media, EDTA Plasma, Hep Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Attractin ELISA Kit
See all Attractin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.1% Inter-assay Sample n Mean SD CV% Serum 3 1% -
Sample type
Saliva, Urine, Serum, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1 ng/ml -
Range
2.81 ng/ml - 180 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 110 101% - 116% Urine 87 85% - 88% Serum 87 78% - 93% Cell culture media 82 78% - 85% Hep Plasma 94 90% - 102% EDTA Plasma 96 95% - 98% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Attractin ELISA Kit (ab263888) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Attractin protein in edta plasma, hep plasma, saliva, serum, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Attractin with 1 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Attractin is involved in the initial clustering of immune cells during inflammatory responses and may play a role in the regulation of chemotactic activity of chemokines. Attractin also plays a critical role in normal myelination in the central nervous system.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Attractin Capture Antibody 1 x 600µl 10X Human Attractin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 5BI 1 x 6ml Denaturant 1 x 500µl Human Attractin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in the initial immune cell clustering during inflammatory response and may regulate chemotactic activity of chemokines. May play a role in melanocortin signaling pathways that regulate energy homeostasis and hair color. Low-affinity receptor for agouti (By similarity). Has a critical role in normal myelination in the central nervous system. -
Tissue specificity
Isoform 2 is detected in plasma (at protein level). Expressed and secreted by activated T lymphocytes. Expressed at low to moderate levels in peripheral blood leukocytes, spleen, lymph node, tonsil, bone marrow and fetal liver. At very low levels found in thymus. Isoform 2 is the major isoform in peripheral blood leukocytes. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 1 CUB domain.
Contains 1 EGF-like domain.
Contains 6 Kelch repeats.
Contains 2 laminin EGF-like domains.
Contains 4 PSI domains. -
Post-translational
modificationsHeavily glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- ATRN
- ATRN_HUMAN
- Attractin
see all -
Database links
- Entrez Gene: 8455 Human
- Omim: 603130 Human
- SwissProt: O75882 Human
- Unigene: 276252 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Attractin standard curve in Sample Diluent NS+Enhancer.The Attractin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Attractin in human serum and plasma samples.The concentrations of Attractin were measured in duplicates, interpolated from the Attractin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (heparin) 1%, and plasma (EDTA) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Attractin concentration was determined to be 2,327 ng/mL in neat serum, 2,638 ng/mL in neat plasma (heparin), and 2,206 ng/mL in neat plasma (EDTA).
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Interpolated concentrations of native Attractin in human saliva and urine samples.The concentrations of Attractin were measured in duplicates, interpolated from the Attractin standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 2% and urine 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Attractin concentration was determined to be 152.4 ng/mL in neat saliva and 203.5 ng/mL in neat urine.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Attractin concentration was determined to be 6,296 ng/mL with a range of 2,868 – 9,858 ng/mL.
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Sandwich ELISA - Human Attractin ELISA Kit (ab263888)To learn more about the advantages of recombinant antibodies see here.
