All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319-26] (ab200353) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : SLC27A4 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HEK-293 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 72 kDa
Observed band size: 72 kDa

Lanes 1-4: Merged signal (red and green). Green - ab200353 observed at 72 kDa. Red - loading control ab8245 observed at 36 kDa.

 ab200353 Anti-SLC27A4 / FATP4 antibody [EPR17319-26] was shown to specifically react with SLC27A4 / FATP4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266114 (knockout cell lysate ab257677) was used. Wild-type and SLC27A4 / FATP4 knockout samples were subjected to SDS-PAGE. ab200353 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes : Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal (ab199719) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : SLC27A4 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HEK-293 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 72 kDa
Observed band size: 72 kDa

Lanes 1-4: Merged signal (red and green). Green - ab199719 observed at 72 kDa. Red - loading control ab8245 observed at 36 kDa.

 ab199719 Anti-SLC27A4 / FATP4 antibody [EPR17319] - C-terminal  was shown to specifically react with SLC27A4 / FATP4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266114 (knockout cell lysate ab257677) was used. Wild-type and SLC27A4 / FATP4 knockout samples were subjected to SDS-PAGE. ab199719 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Homozygous: 14 bp deletion in exon 2

Representative images of SLC27A4 knockout HEK293T cells, low and high confluency examples (top left and right respectively) and wild-type HEK293T cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using an EVOS M5000 microscope.