Anti-ULBP2 + RAET1G antibody [EPR24077-5] - BSA and Azide free (ab280890)
![Anti-ULBP2 + RAET1G antibody [EPR24077-5] - BSA and Azide free (ab280890)](https://www.abcam.com/ps/products/280/ab280890/Images/ab280890-456165-anti-ulbp5ulbp2-antibody-epr240775-western-blot-myelogenous.jpg "Anti-ULBP2 + RAET1G antibody [EPR24077-5] - BSA and Azide free (ab280890)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24077-5] to ULBP2 + RAET1G - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-ULBP2 + RAET1G antibody [EPR24077-5] - BSA and Azide free -
Description
Rabbit monoclonal [EPR24077-5] to ULBP2 + RAET1G - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,Flow Cyt (Intra),ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged human ULBP1 recombinant protein, FC-tagged human ULBP2 recombinant protein, His-tagged human ULBP5 recombinant protein, His-tagged human ULBP6 recombinant protein, K562, NIH:OVCAR-3, Jurkat, HepG2, THP-1 , Human ovary cancer lysates.
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General notes
ab280890 is the carrier-free version of ab259955. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab280890 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24077-5 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ULBP2 + RAET1G antibody [EPR24077-5] - BSA and Azide free (ab280890)All lanes : Anti-ULBP2 + RAET1G antibody [EPR24077-5] (ab259955) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 2 : NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 4 : HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate 20
Lane 5 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
Lane 6 : Human ovary tissue lysate
Lane 7 : Human ovary cancer tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Observed band size: 37 kDa why is the actual band size different from the predicted?This data was developed using ab259955, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Human ovary(PMID: 16914326; PMID: 18791713; PMID: 19861434)
Exposure time: 3 minutes
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![Western blot - Anti-ULBP2 + RAET1G antibody [EPR24077-5] - BSA and Azide free (ab280890)](https://www.abcam.com/ps/products/280/ab280890/Images/ab280890-456164-anti-ulbp5ulbp2-antibody-epr240775-western-blot-recombinant.jpg)
Western blot - Anti-ULBP2 + RAET1G antibody [EPR24077-5] - BSA and Azide free (ab280890)All lanes : Anti-ULBP2 + RAET1G antibody [EPR24077-5] (ab259955) at 1/1000 dilution
Lane 1 : His-tagged human ULBP1 recombinant protein (aa 29-216)
Lane 2 : FC-tagged human ULBP2 recombinant protein (aa 26-217)
Lane 3 : His-tagged human ULBP5 recombinant protein (aa 29-210)
Lane 4 : His-tagged human ULBP6 recombinant protein (aa 26-218)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Observed band size: 25-75 kDa why is the actual band size different from the predicted?This data was developed using ab259955, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
We have preliminary internal testing data to indicate this antibody weakly cross reacts with recombinant human ULBP1/6 protein in WB.
Exposure time: 7.75 seconds
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![Anti-ULBP2 + RAET1G antibody [EPR24077-5] - BSA and Azide free (ab280890)](https://www.abcam.com/ps/products/280/ab280890/Images/ab280890-3-Benefits-of-Recombinant-antibodies.jpg)
Anti-ULBP2 + RAET1G antibody [EPR24077-5] - BSA and Azide free (ab280890)