Anti-Tyrosine Hydroxylase antibody (ab137721)
Key features and details
- Rabbit polyclonal to Tyrosine Hydroxylase
- Suitable for: IHC-FoFr, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Tyrosine Hydroxylase antibody
See all Tyrosine Hydroxylase primary antibodies -
Description
Rabbit polyclonal to Tyrosine Hydroxylase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-FoFr, WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 150-490 of Human Tyrosine Hydroxylase.
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Positive control
- NT2D1, PC-3, U87-MG, SK-N-SH, Mouse brain and PC-12 whole cell lysates; SK-N-SH cells; Human H661 xenograft and Rat hindlimb pad skin tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)Immunohistochemical analysis of paraffin-embedded H661 xenograft labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.
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Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg
Predicted band size: 58 kDa
7.5% SDS-PAGE -
Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)All lanes : Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/500 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : PC-3 whole cell lysate
Lane 3 : U87-MG whole cell lysate
Lane 4 : SK-N-SH whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
7.5 % SDS-PAGE -
Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/500 dilution + Mouse brain lysate at 50 µg
Predicted band size: 58 kDa
7.5 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab137721)Immunofluorescent analysis of methanol-fixed SK-N-SH cells labelling Tyrosine Hydroxylase with ab137721 at 1/500 dilution (left panel) and co-stained with Hoechst 33343 (right panel). SK-N-SH cells were fixed in -20°C 100% MeOH for 5 min. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)Immunohistochemical analysis of paraformaldehyde-fixed frozen sections of Rat hindlimb pad skin tissue labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)Immunohistochemical analysis of paraformaldehyde-fixed frozen sections of Rat hindlimb pad skin tissue labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.
