Anti-Twist2 antibody (ab66031)
Key features and details
- Rabbit polyclonal to Twist2
- Suitable for: WB, IP
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Twist2 antibody
See all Twist2 primary antibodies -
Description
Rabbit polyclonal to Twist2 -
Host species
Rabbit -
Specificity
This antibody is able to detect both Twist1 and Twist2 proteins. We have observed weak detection of the Twist1 protein using Twist1 recombinant proteins along with select lysates generating a BOI of weak signal associated to Twist1. The signal detected in the endogenous lysates and recombinant proteins is much greater for Twist2. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment
Predicted to work with: Rat, Chicken, Cow
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Immunogen
Synthetic peptide corresponding to Human Twist2 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab86241) -
Positive control
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab66031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). IP Use a concentration of 5 µg/ml. Target
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Function
Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism (By similarity). Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. -
Tissue specificity
In the embryo, highly expressed in chondrogenic cells. In embryonic skin, expressed in the undifferentiated mesenchymal layer beneath the epidermis which later develops into the dermis. Expressed in early myeloid cells but not in lymphoid cells in the liver. Expression also detected in the secretory ependymal epithelium of the choroid plexus primordium. In the adult, expressed in secreting glandular tissues and tubules. -
Involvement in disease
Defects in TWIST2 are the cause of Setleis syndrome (SETLEISS) [MIM:227260]. A focal facial dermal dysplasia characterized by distinctive bitemporal scar-like depressions resembling forceps marks, and additional facial features, including a coarse and leonine appearance, absent eyelashes on both lids or multiple rows on the upper lids, absent Meibomian glands, slanted eyebrows, chin clefting, and hypo- or hyperpigmentation of the skin. Histologically, the bitemporal lesion is an ectodermal dysplasia with near absence of subcutaneous fat, suggesting insufficient migration of neural crest cells into the frontonasal process and the first branchial arch. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear during embryonic development. Cytoplasmic in adult tissues. - Information by UniProt
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Database links
- Entrez Gene: 117581 Human
- Entrez Gene: 13345 Mouse
- Entrez Gene: 59327 Rat
- Omim: 607556 Human
- SwissProt: Q8WVJ9 Human
- SwissProt: Q9D030 Mouse
- SwissProt: P97831 Rat
- Unigene: 422585 Human
see all -
Alternative names
- bHLHa39 antibody
- Class A basic helix-loop-helix protein 39 antibody
- Dermis expressed protein 1 antibody
see all
Images
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Western blot - Anti-Twist2 antibody (ab66031)All lanes : Anti-Twist2 antibody (ab66031) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Additional bands at: 24 kDa, 55 kDa, 90 kDa. We are unsure as to the identity of these extra bands.This antibody is able to detect both Twist1 and Twist2 proteins. However the affinity for Twist2 is much greater. The BOI at 18kDa is related to the higher affinity binding of Twist2. In select lysates, we have observed a weaker band between 21-25kDa likely to be the cross reactivity of Twist1.
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Western blot - Anti-Twist2 antibody (ab66031)All lanes : ab66031 at 1 µg/ml
Lane 1 : Human liver tissue lysate
Lane 2 : Human HEK293 whole cell lysate
Lane 3 : Human T47D whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 3000 cells
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Additional bands at: 21 kDa (possible cross reactivity), 45 kDa, 46 kDa (possible non-specific binding). We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteThis antibody is able to detect both Twist1 and Twist2 proteins. However the affinity for Twist2 is much greater. The BOI at 18kDa is related to the higher affinity binding of Twist2. In select lysates, we have observed a weaker band between 21-25kDa likely to be the cross reactivity of Twist1.
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Immunoprecipitation - Anti-Twist2 antibody (ab66031)Twist2 was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Rabbit polyclonal to Twist2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab66031.
Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
Band: 18kDa; Twist2 -
Western blot - Anti-Twist2 antibody (ab66031)All lanes : ab66031 at 1 µg/ml
Lane 1 : Recombinant Human Twist2 protein ab132641
Lane 2 : Recombinant Human Twist2 protein ab153585
Lane 3 : Recombinant Human Twist protein ab112368
Lane 4 : Recombinant Human Twist protein ab132349
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 37,45,48 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis antibody is able to detect both Twist1 and Twist2 proteins. However the affinity for Twist2 is much greater, as demonstrated by the use of the recombinant proteins.
Protocols
Datasheets and documents
References (12)
ab66031 has been referenced in 12 publications.
- Xu R et al. MicroRNA-940 inhibits epithelial-mesenchymal transition of glioma cells via targeting ZEB2. Am J Transl Res 11:7351-7363 (2019). PubMed: 31934283
- Kou B et al. HMGA2 facilitates epithelial-mesenchymal transition in renal cell carcinoma by regulating the TGF-ß/Smad2 signaling pathway. Oncol Rep 39:101-108 (2018). PubMed: 29138866
- Yang J et al. HIF-2a promotes the formation of vasculogenic mimicry in pancreatic cancer by regulating the binding of Twist1 to the VE-cadherin promoter. Oncotarget 8:47801-47815 (2017). PubMed: 28599281
- Li H et al. Insulin-like growth factor-I induces epithelial to mesenchymal transition via GSK-3ß and ZEB2 in the BGC-823 gastric cancer cell line. Oncol Lett 9:143-148 (2015). PubMed: 25435948
- Zheng S et al. Twist1 and Twist2 Contribute to Cytokine Downregulation following Chronic NOD2 Stimulation of Human Macrophages through the Coordinated Regulation of Transcriptional Repressors and Activators. J Immunol 195:217-26 (2015). ChIP ; Human . PubMed: 26019273
Images
-
Western blot - Anti-Twist2 antibody (ab66031)All lanes : Anti-Twist2 antibody (ab66031) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Additional bands at: 24 kDa, 55 kDa, 90 kDa. We are unsure as to the identity of these extra bands.This antibody is able to detect both Twist1 and Twist2 proteins. However the affinity for Twist2 is much greater. The BOI at 18kDa is related to the higher affinity binding of Twist2. In select lysates, we have observed a weaker band between 21-25kDa likely to be the cross reactivity of Twist1.
-
Western blot - Anti-Twist2 antibody (ab66031)All lanes : ab66031 at 1 µg/ml
Lane 1 : Human liver tissue lysate
Lane 2 : Human HEK293 whole cell lysate
Lane 3 : Human T47D whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 3000 cells
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Additional bands at: 21 kDa (possible cross reactivity), 45 kDa, 46 kDa (possible non-specific binding). We are unsure as to the identity of these extra bands.
Exposure time: 1 minuteThis antibody is able to detect both Twist1 and Twist2 proteins. However the affinity for Twist2 is much greater. The BOI at 18kDa is related to the higher affinity binding of Twist2. In select lysates, we have observed a weaker band between 21-25kDa likely to be the cross reactivity of Twist1.
-
Immunoprecipitation - Anti-Twist2 antibody (ab66031)Twist2 was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Rabbit polyclonal to Twist2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab66031.
Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
Band: 18kDa; Twist2 -
Western blot - Anti-Twist2 antibody (ab66031)All lanes : ab66031 at 1 µg/ml
Lane 1 : Recombinant Human Twist2 protein ab132641
Lane 2 : Recombinant Human Twist2 protein ab153585
Lane 3 : Recombinant Human Twist protein ab112368
Lane 4 : Recombinant Human Twist protein ab132349
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 37,45,48 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis antibody is able to detect both Twist1 and Twist2 proteins. However the affinity for Twist2 is much greater, as demonstrated by the use of the recombinant proteins.
