Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-9 antibody [EPR23484-151] (ab227037)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23484-151] to IL-9
  • Suitable for: ICC/IF, WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-IL-9 antibody [EPR23484-151]
    See all IL-9 primary antibodies

  • Description

    Rabbit monoclonal [EPR23484-151] to IL-9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged mouse IL9 recombinant protein, Mouse and rat spleen, thymus lysates. ICC/IF: 293T cell. Flow Cyt: 293T cell. IP: Mouse spleen lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23484-151

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037)
    Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037)
    All lanes : Anti-IL-9 antibody [EPR23484-151] (ab227037) at 1/1000 dilution

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Rat thymus tissue lysate

    Lysates/proteins at 60 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 15 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9806735).

    Exposure time: 104 seconds.

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-9 antibody [EPR23484-151] (ab227037)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-9 antibody [EPR23484-151] (ab227037)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with HA tagged mouse IL9 construct cells labelling IL-9 with ab227037 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing strong membranous and cytoplasmic staining in 293T cells transfected with HA tagged mouse IL9 construct. anti-HA.11 Epitope Tag mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.

  • Flow Cytometry - Anti-IL-9 antibody [EPR23484-151] (ab227037)
    Flow Cytometry - Anti-IL-9 antibody [EPR23484-151] (ab227037)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) transfected with HA tagged mouse IL9 construct cells labelling IL-9 with ab227037 at 1/500 dilution (0.1µg) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

     

  • Immunoprecipitation - Anti-IL-9 antibody [EPR23484-151] (ab227037)
    Immunoprecipitation - Anti-IL-9 antibody [EPR23484-151] (ab227037)

    IL-9 was immunoprecipitated from 0.35 mg mouse spleen tissue lysate 10µg with ab227037 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab227037 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse spleen tissue lysate 10µg

    Lane 2: ab227037 IP in mouse spleen tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227037 in mouse spleen tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037)
    Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037)
    All lanes : Anti-IL-9 antibody [EPR23484-151] (ab227037) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Mouse thymus tissue lysate

    Lysates/proteins at 60 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 15 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9806735).

    Exposure time: 3 minutes.

  • Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037)
    Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037)
    Anti-IL-9 antibody [EPR23484-151] (ab227037) at 1/1000 dilution + His-tagged mouse IL9 recombinant protein, 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 15 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9806735).

    Exposure time: 10 seconds.

  • Anti-IL-9 antibody [EPR23484-151] (ab227037)
    Anti-IL-9 antibody [EPR23484-151] (ab227037)

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